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An intensive and comprehensive laboratory-oriented course in cellular and molecular neurobiology intended for predoctoral students, postdoctoral or clinical researchers, and young investigators beginning independent research careers. Limited to 12 students.
A hallmark of this course is the extensive lab work done in close
collaboration with expert faculty. The course is divided into three
sections: Electrophysiology, Imaging, and Molecular Neurobiology.
These are taught by separate groups of faculty, usually six in each
section, and with many guest lecturers. Each section begins with specific
training in core laboratory techniques; students then undertake one-
to two-week directed or independent projects using the methods they
have learned. Didactic lectures are combined with laboratory experience
in order to establish a strong conceptual foundation for each section.
A typical day has 3 hours of lecture and 10 hours of lab.
Electrophysiological methods focus on patch-clamp and sharp electrode recordings, performed on neurons in a variety of preparations, including tissue culture, brain slices, isolated squid synapses, rat cochlea, or whole fish. Optical methods include calcium imaging, confocal and 2-photon microscopy, videomicroscopy, and electron microscopy. Molecular techniques emphasize the use of forward and reverse genetics in diverse systems such as Drosophila, C. elegans, zebrafish, chick embryos, and primary cells in culture. The impact of genetic manipulations are assayed by real time PCR, laser microdissection, single cell PCR, in situ hybridization, and a variety of immunotechniques in addition to incorporating electrophysiological and imaging techniques.
The goal of the course is to emphasize the strengths of a multidisciplinary approach for studying the function of the nervous system at the cellular and molecular levels.
This course is supported in part by grants from the National Institute of Neurological Disorders and Stroke, the National Institute of Mental Health, and the Grass Foundation.
2008 Faculty and Lecturers
Susan Ackerman, The Jackson Laboratory
Ricardo Araneda, University of Maryland
Christine Beattie, The Ohio State University
Francisco Bezanilla, University of Chicago
JoAnn Buchanan, Stanford University
Ingrid Bureau, INSERM
Ann-Shyn Chiang, National Tsing Hua University
Andrew Chisholm, University of California, San Diego
Dmitri Chklovskii, Howard Hughes Medical Institute
Thomas Clandinin, Stanford University
David Clapham, Children's Hospital, Harvard Medical School
Lawrence Cohen, Yale University
Kathryn Commons, Children's Hospital/Harvard Medical
Matthew Dalva, University of Pennsylvania
Karl Deisseroth, Stanford University
Elva Diaz, University of California, Davis
Ruth Anne Eatock, Massachusetts Eye & Ear Infirmary
Florian Engert, Harvard University
Ying-Hui Fu, University of California, San Francisco
Kurt Haas, University of British Columbia
Kristen Harris, University of Texas, Austin
Michael Hausser, University College London
Paul Henion, The Ohio State University
Soren Impey, Oregon Health & Science University
Carlota Gonzalez Inchauspe, IFIBYNE
Erik Jorgensen, University of Utah, HHMI
Eleonora Katz, INGEBI
Julie Kauer, Brown University
Darcy Kelley, Columbia University
Jeff Lichtman, Harvard University
John Lipscombe, Brown University
Isabel Llano, CNRS
Roderick Mackinnon, The Rockefeller University, HHMI
Gail Mandel, Oregon Health & Science University, HHMI
Andres Maricq, University of Utah
Alain Marty, CNRS
Kristina Micheva, Stanford University
Christopher Miller, Brandeis University
Thomas Misgeld, Technical University Munich
Venkatesh Murthy, Harvard University
Thomas Oertner, Friedrich Miescher Institute
Louis Ptacek, University of California, San Francisco, HHMI
Christian Rosenmund, Baylor College of Medicine
Edward Ruthazer, McGill University
Jon Sack, Institute for Design of Intelligent Drugs
Josh Sanes, Harvard University
Peter Scheiffele, Columbia University
Dietmar Schmucker, Harvard Medical School
Stephen Smith, Stanford University
Michael Szulczewski, Prairie Technologies, Inc.
Osvaldo Uchitel, IFIBYNE
Ryohei Yasuda, Duke University Medical Center
Yi Zuo, UC Santa Cruz
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